Needle-shaped beam optical-resolution photoacoustic microscopy with an extended depth of field
Abstract
To extend the depth of field (DOF) in optical-resolution photoacoustic microscopy (OR-PAM), we propose the needle-shaped beam photoacoustic microscopy (NB-PAM) via customized diffractive optical elements to extend the DOF, featuring a well-maintained beam diameter, a uniform axial intensity distribution, and negligible sidelobes. The advantage of using NB-PAM with an improved DOF has been demonstrated by both histology-like imaging of fresh slide-free organs using 266 nm laser and in vivo mouse brain vasculature imaging using 532 nm laser. Our approach provides new perspectives for slide-free intraoperative pathological imaging and various in vivo organ-level imaging applications.
Yide Zhang
NIH K99 Postdoctoral Fellow
My research is interdisciplinary and focused on developing new types of optical imaging techniques that could advance the work of other researchers and medical personnel in a wide variety of fields. Currently, I am developing next-generation photoacoustic and ultrafast imaging techniques that can observe biological and physical phenomena that are too fast to be imaged with existing methods. The observation of the ultrafast phenomena could provide a better understanding of the fundamentals of life and physical sciences. I am also developing novel quantum imaging approaches that can investigate biological organisms with an imaging performance that cannot be achieved using classical optical imaging. In my free time, I enjoy cooking, hiking, cycling, and traveling.