Stepwise optical saturation microscopy: obtaining super-resolution images with conventional fluorescence microscopes

Abstract

We propose stepwise optical saturation (SOS) microscopy, in which two conventional fluorescence images are linearly combined to extend the resolution by 41%. We experimentally perform the SOS microscopy with one-photon (confocal) and multiphoton excitation fluorescence.

Publication
Biomedical Optics 2018, Hollywood, Florida USA
Yide Zhang
Yide Zhang
Postdoctoral Scholar

My research is interdisciplinary and focused on developing new types of optical imaging techniques that could advance the work of other researchers and medical personnel in a wide variety of fields. Currently, I am developing next-generation photoacoustic and ultrafast imaging techniques that can observe biological and physical phenomena that are too fast to be imaged with existing methods. The observation of the ultrafast phenomena could provide a better understanding of the fundamentals of life and physical sciences. I am also developing novel quantum imaging approaches that can investigate biological organisms with an imaging performance that cannot be achieved using classical optical imaging. In my free time, I enjoy cooking, hiking, cycling, and traveling.

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