Super-resolution

Enhancing optical microscopy with quantum entanglement

Drawing on the unique properties of entangled photons, quantum approaches can overcome the limitations of classical methods, enhance spatial resolution and reduce stray light and shot noise.

Quantum imaging of biological organisms using hyperentangled photon pairs

We introduce quantum imaging by coincidence from entanglement (ICE) that overcomes challenges faced by existing quantum imaging methods. ICE utilizes hyperentangled photon pairs to achieve higher signal-to-noise ratios, greater resolvable pixel …

Super-resolution quantum microscopy at the Heisenberg limit

We introduce quantum microscopy by coincidence (QMC) featuring balanced pathlengths, which facilitates super-resolution imaging at the Heisenberg limit, drastically boosting speed and contrast-to-noise ratio (CNR) compared to existing wide-field …

Quantum imaging of biological organisms through spatial and polarization entanglement

Quantum imaging holds potential benefits over classical imaging but has faced challenges such as poor signal-to-noise ratios, low resolvable pixel counts, difficulty in imaging biological organisms, and inability to quantify full birefringence …

Quantum microscopy of cells at the Heisenberg limit

Entangled biphoton sources exhibit nonclassical characteristics and have been applied to imaging techniques such as ghost imaging, quantum holography, and quantum optical coherence tomography. The development of wide-field quantum imaging to date has …

Instant FLIM enables 4D in vivo lifetime imaging of intact and injured zebrafish and mouse brains

Traditional fluorescence microscopy is blind to molecular microenvironment information that is present in a fluorescence lifetime, which can be measured by fluorescence lifetime imaging microscopy (FLIM). However, most existing FLIM techniques are …

Deep learning-based super-resolution fluorescence microscopy on small datasets

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to resolve. While …

Overcoming the fundamental limitation of frequency-domain fluorescence lifetime imaging microscopy spatial resolution

We propose and demonstrate the first analytical model of the spatial resolution of frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM) that explains how it is fundamentally different with the common resolution limit of the …

Resolution enhancement of transmission electron microscopy by super-resolution radial fluctuations

Super-resolution fluorescence microscopy techniques have enabled dramatic development in modern biology due to their capability to discern features smaller than the diffraction limit of light. Recently, super-resolution radial fluctuations (SRRF), an …

Generating intravital super-resolution movies with conventional microscopy reveals actin dynamics that construct pioneer axons

Super-resolution microscopy is broadening our in-depth understanding of cellular structure. However, super-resolution approaches are limited, for numerous reasons, from utilization in longer-term intravital imaging. We devised a combinatorial imaging …