PAT

Sharp-peaked lanthanide nanocrystals for near-infrared photoacoustic multiplexed differential imaging

Photoacoustic tomography offers a powerful tool to visualize biologically relevant molecules and understand processes within living systems at high resolution in deep tissue, facilitated by the conversion of incident photons into low-scattering …

Ultrafast single-shot 3D photoacoustic tomography in vivo using a single-element detector

We present photoacoustic computed tomography through an ergodic relay (PACTER), a method for single-shot 3D imaging of hemodynamics using a single-element detector. Our approach allows for ultrafast volumetric imaging at kilohertz rates without the …

Ultrafast longitudinal imaging of haemodynamics via single-shot volumetric photoacoustic tomography with a single-element detector

Techniques for imaging haemodynamics use ionizing radiation or contrast agents or are limited by imaging depth (within approximately 1 mm), complex and expensive data-acquisition systems, or low imaging speeds, system complexity or cost. Here we show …

Non-invasive photoacoustic computed tomography of cardiac anatomy and function in rats

Combining functional optical contrast with high spatiotemporal resolution, photoacoustic computed tomography (PACT) benefits mainstream cardiac imaging modalities for preclinical research. However, PACT has not revealed detailed vasculature or …

Needle-shaped beam optical-resolution photoacoustic microscopy with an extended depth of field

To extend the depth of field (DOF) in optical-resolution photoacoustic microscopy (OR-PAM), we propose the needle-shaped beam photoacoustic microscopy (NB-PAM) via customized diffractive optical elements to extend the DOF, featuring a well-maintained …

Non-invasive photoacoustic computed tomography of rat heart anatomy and function

Complementary to mainstream cardiac imaging modalities for preclinical research, photoacoustic computed tomography (PACT) can provide functional optical contrast with high imaging speed and resolution. However, PACT has not been demonstrated to …

Optical-resolution photoacoustic microscopy with a needle-shaped beam

Optical-resolution photoacoustic microscopy can visualize wavelength-dependent optical absorption at the cellular level. However, this technique suffers from a limited depth of field due to the tight focus of the optical excitation beam, making it …

Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy

Obtaining frozen sections of bone tissue for intraoperative examination is challenging. To identify the bony edge of resection, orthopaedic oncologists therefore rely on pre-operative X-ray computed tomography or magnetic resonance imaging. However, …

Label-free ultraviolet photoacoustic histology via deep learning for rapid intraoperative diagnosis of bone cancer

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Snapshot photoacoustic topography through an ergodic relay of optical absorption in vivo

Photoacoustic tomography (PAT) has demonstrated versatile biomedical applications, ranging from tracking single cells to monitoring whole-body dynamics of small animals and diagnosing human breast cancer. Currently, PAT has two major implementations: …