We propose and demonstrate a novel multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) system that is able to generate 3D lifetime images in deep scattering tissues. The imaging speed of FD-FLIM is improved using phase …
We present the first experimental demonstration of super-resolution multiphoton frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM). This is obtained through a novel microscopy technique called generalized stepwise optical …
We present a novel super-resolution fluorescence lifetime microscopy technique called generalized stepwise optical saturation (GSOS) that generalizes and extends the concept of the recently demonstrated stepwise optical saturation (SOS) …
In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, …
We propose stepwise optical saturation (SOS) microscopy, in which two conventional fluorescence images are linearly combined to extend the resolution by 41%. We experimentally perform the SOS microscopy with one-photon (confocal) and multiphoton …
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy …
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high …
Here we recount the standard two-level model that describes saturated excitation (SAX) in multiphoton microscopy (MPM), a new technique for super-resolution fluorescence microscopy in scattering tissue, which requires no special chemistry and only …
We demonstrate super-sensitive multiphoton frequency-domain fluorescence lifetime images using the recently developed DC&1ω method by showing a two-fold improvement in imaging sensitivity compared to the conventional 1ω phase fluorometry.
SAX is modeled and found to generate irregular PSF containing spatial frequency content beyond the diffraction limit. No special chemistry and minimal modification of MPM is needed toward super-resolved fluorescence imaging in scattering media.