MPM

Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics

We propose and demonstrate a novel multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) system that is able to generate 3D lifetime images in deep scattering tissues. The imaging speed of FD-FLIM is improved using phase …

Super-resolution multiphoton frequency-domain fluorescence lifetime imaging microscopy by generalized stepwise optical saturation (GSOS)

We present the first experimental demonstration of super-resolution multiphoton frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM). This is obtained through a novel microscopy technique called generalized stepwise optical …

Generalized stepwise optical saturation enables super-resolution fluorescence lifetime imaging microscopy

We present a novel super-resolution fluorescence lifetime microscopy technique called generalized stepwise optical saturation (GSOS) that generalizes and extends the concept of the recently demonstrated stepwise optical saturation (SOS) …

Super-resolution and high-speed quantitative multiphoton microscopy

In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, …

Stepwise optical saturation microscopy: obtaining super-resolution images with conventional fluorescence microscopes

We propose stepwise optical saturation (SOS) microscopy, in which two conventional fluorescence images are linearly combined to extend the resolution by 41%. We experimentally perform the SOS microscopy with one-photon (confocal) and multiphoton …

Super-resolution fluorescence microscopy by stepwise optical saturation

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy …

Super-resolution fluorescence imaging by stepwise optical saturation microscopy

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high …

Description of deep saturated excitation multiphoton microscopy for super-resolution imaging

Here we recount the standard two-level model that describes saturated excitation (SAX) in multiphoton microscopy (MPM), a new technique for super-resolution fluorescence microscopy in scattering tissue, which requires no special chemistry and only …

Doubling the sensitivity of multiphoton frequency-domain fluorescence lifetime images

We demonstrate super-sensitive multiphoton frequency-domain fluorescence lifetime images using the recently developed DC&1ω method by showing a two-fold improvement in imaging sensitivity compared to the conventional 1ω phase fluorometry.

Simulation and experimental design of saturated excitation (SAX) multiphoton microscopy (MPM)

SAX is modeled and found to generate irregular PSF containing spatial frequency content beyond the diffraction limit. No special chemistry and minimal modification of MPM is needed toward super-resolved fluorescence imaging in scattering media.