FLIM

Editorial: Phasor analysis for fluorescence lifetime data

Fluorescence decay kinetic data provide significant and additional insight for many biological, biomedical, biochemical, and biophysical studies. The fluorescence lifetime is a valuable measurement because of the quantitative information that is …

Instant FLIM enables 4D in vivo lifetime imaging of intact and injured zebrafish and mouse brains

Traditional fluorescence microscopy is blind to molecular microenvironment information that is present in a fluorescence lifetime, which can be measured by fluorescence lifetime imaging microscopy (FLIM). However, most existing FLIM techniques are …

Convolutional neural network denoising in fluorescence lifetime imaging microscopy (FLIM)

Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising convolutional …

Overcoming the fundamental limitation of frequency-domain fluorescence lifetime imaging microscopy spatial resolution

We propose and demonstrate the first analytical model of the spatial resolution of frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM) that explains how it is fundamentally different with the common resolution limit of the …

Machine learning for faster and smarter fluorescence lifetime imaging microscopy

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis, and …

Automatic segmentation of intravital fluorescence microscopy images by K-means clustering of FLIM phasors

Fluorescence lifetime imaging microscopy (FLIM) provides additional contrast for fluorophores with overlapping emission spectra. The phasor approach to FLIM greatly reduces the complexity of FLIM analysis and enables a useful image segmentation …

Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics

We propose and demonstrate a novel multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) system that is able to generate 3D lifetime images in deep scattering tissues. The imaging speed of FD-FLIM is improved using phase …

Super-resolution multiphoton frequency-domain fluorescence lifetime imaging microscopy by generalized stepwise optical saturation (GSOS)

We present the first experimental demonstration of super-resolution multiphoton frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM). This is obtained through a novel microscopy technique called generalized stepwise optical …

Generalized stepwise optical saturation enables super-resolution fluorescence lifetime imaging microscopy

We present a novel super-resolution fluorescence lifetime microscopy technique called generalized stepwise optical saturation (GSOS) that generalizes and extends the concept of the recently demonstrated stepwise optical saturation (SOS) …

Super-resolution and high-speed quantitative multiphoton microscopy

In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, …