SNR

Doubling the sensitivity of multiphoton frequency-domain fluorescence lifetime images

We demonstrate super-sensitive multiphoton frequency-domain fluorescence lifetime images using the recently developed DC&1ω method by showing a two-fold improvement in imaging sensitivity compared to the conventional 1ω phase fluorometry.

Super-sensitivity and super-resolution multiphoton fluorescence lifetime imaging microscopy

To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform …

Super-sensitivity multiphoton frequency-domain fluorescence lifetime imaging microscopy

We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the …

Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy

Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging microscopy (FLIM) has enabled three-dimensional quantitative molecular microscopy in vivo. The signal-to-noise ratio (SNR), and thus the imaging rate of MPM-FLIM, which is …

Theoretical analysis of the signal-to-noise ratio of two-photon oxygen imaging probes

Signal-to-noise ratio performance of optical chemical sensors in the presence of phosphorescence saturation is described using Poisson statistics and Stern-Volmer kinetics. The described framework can be applied across a wide range of sensitivites …

On increasing the imaging rate of frequency-domain multiphoton fluorescence lifetime imaging microscopy

Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large …