Super-resolution microscopy is broadening our in-depth understanding of cellular structure. However, super-resolution approaches are limited, for numerous reasons, from utilization in longer-term intravital imaging. We devised a combinatorial imaging …
We present a novel super-resolution fluorescence lifetime microscopy technique called generalized stepwise optical saturation (GSOS) that generalizes and extends the concept of the recently demonstrated stepwise optical saturation (SOS) …
In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, …
We propose stepwise optical saturation (SOS) microscopy, in which two conventional fluorescence images are linearly combined to extend the resolution by 41%. We experimentally perform the SOS microscopy with one-photon (confocal) and multiphoton …
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the super-resolution microscopy …
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high …
Here we recount the standard two-level model that describes saturated excitation (SAX) in multiphoton microscopy (MPM), a new technique for super-resolution fluorescence microscopy in scattering tissue, which requires no special chemistry and only …
SAX is modeled and found to generate irregular PSF containing spatial frequency content beyond the diffraction limit. No special chemistry and minimal modification of MPM is needed toward super-resolved fluorescence imaging in scattering media.
Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain …