MPM

Silica-coated ruthenium-complex nanoprobes for two-photon oxygen microscopy in biological media

Multiphoton microscopy (MPM) allows for three-dimensional in vivo microscopy in scattering tissue with submicron resolution and high signal-to-noise ratio. MPM combined with fluorescence lifetime measurements further enables quantitative imaging of …

Super-sensitivity and super-resolution multiphoton fluorescence lifetime imaging microscopy

To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform …

Super-sensitivity multiphoton frequency-domain fluorescence lifetime imaging microscopy

We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the …

Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy

Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging microscopy (FLIM) has enabled three-dimensional quantitative molecular microscopy in vivo. The signal-to-noise ratio (SNR), and thus the imaging rate of MPM-FLIM, which is …

High-resolution frequency-domain multiphoton fluorescence lifetime imaging microscopy

Multiphoton microscopy (MPM) is a widely used in vivo imaging technique in biological and medical applications. In the case of two-photon excitation fluorescence, two lower-energy photons excite a fluorophore which in turn emits a single …

On increasing the imaging rate of frequency-domain multiphoton fluorescence lifetime imaging microscopy

Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large …