Frequency-domain

Overcoming the fundamental limitation of frequency-domain fluorescence lifetime imaging microscopy spatial resolution

We propose and demonstrate the first analytical model of the spatial resolution of frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM) that explains how it is fundamentally different with the common resolution limit of the …

Self-calibrated frequency domain diffuse optical spectroscopy with a phased source array

In this paper, we show that interfering multiple photon density waves created by intensity-modulated sources in frequency domain diffuse optical spectroscopy (fd-DOS) can be used to recover the optical properties of homogenous and heterogeneous …

Saturation-compensated measurements for fluorescence lifetime imaging microscopy

Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain …

Super-sensitivity multiphoton frequency-domain fluorescence lifetime imaging microscopy

We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the …

Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy

Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging microscopy (FLIM) has enabled three-dimensional quantitative molecular microscopy in vivo. The signal-to-noise ratio (SNR), and thus the imaging rate of MPM-FLIM, which is …